Electronic Journal of Biotechnology ISSN: 0717-3458
  © 2001 by Universidad Católica de Valparaíso -- Chile
Vol. 4 No. 1, Issue of April 15, 2001
 


Table 1. Purification of CGRP like molecules obtained from sardine hydrolysates.

Proteins, g

CGRP-like immuno reactivity, pg

Specific activitya, pg/g protein

Purification factorb

RRA ED50, g/tubec

Crude extract

22,000

30

1.4 10-4

311

Gel Exclusion

250

16

0.06

46

101

HPLC 1

54

23

0.42

304

7.3

HPLC 2

14

245

18

12500

0.9

a The specific activity represents the quantity of CGRP like molecules per g of protein.
b The purification factor is the ratio of the specific activity of the crude extract to that obtained at each purification step.
c Data were expressed as the quantity of proteins (g) that induced a 50% inhibition of the initial CGRP binding to rat liver membranes.

 

 

 


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