Electronic Journal of Biotechnology ISSN: 0717-3458
  © 2001 by Universidad Católica de Valparaíso -- Chile
Published by Ejbiotechnology


Figure 3. Second HPLC chromatography. The CGRP like molecules obtained from the first HPLC chromatography were further purified on the same HPLC column using a linear gradient from 30 to 60% acetonitrile in 0.1% TFA. The line shows the concentration of acetonitrile in the eluting solvent. Optical density was measured at 226 nm (¨) and the CGRP Iike immunoreactivity (l) determined on an aliquot from each fraction.

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