Electronic Journal of Biotechnology ISSN: 0717-3458
  © 2000 by Universidad Católica de Valparaíso -- Chile
Vol. 3 No. 1, Issue of April 15, 2000
 

 

Table 1. Background and MNNG-induced mutation frequencies in the supF sequence of pS189 shuttle vector plasmids.Mutation Frequency (%)

Host Cells

MNNG

# of mutant colonies/# of total colonies

Mutation Frequency (%)

NHOK

-

13/25,406

0.051

NHOK

+

33/20,290

0.163

HOK/LXSN

-
12/18,878
0.064

HOK/LXSN

+
37/24,177
0.153

HOK/16E6

-

30/17,294

0.173a

HOK/16F6

+
105/17,764
0.591b

HOK/16E7

-
22/18,665
0.118a

HOK/16E7

+

90/21,846

0.412b

HOK/16E6/E7

-
33/18,660
0.177a

HOK/16F6/E7

+
123/18,769
0.655b

HOK/6bE6

-

18/19,504

0.092c

HOK/6bE6

+
35/15,368
0.228

HOK/6bE7

-
10/18,972
0.053

HOK/6bE7

+
31/18,843
0.165

 

a Significantly different from NHOK (-) group (Binomial comparison of two Poisson rates)

b Significantly different from NHOK (+) group (Binomial comparison).

c Not significantly different from NHOK(-) group (Binomial comparison).

Cells transfected with pS189 were exposed to MNNG (1.0 m g/ml) for 2 hours and incubated in fresh medium for 24 hours. The pS189 plasmids were recovered and intro