Electronic Journal of Biotechnology ISSN: 0717-3458
  © 2000 by Universidad Católica de Valparaíso -- Chile
Vol. 3 No. 1, Issue of April 15, 2000
 

 

Table 1. Mutation frequencies of pS189 shuttle vector plasmid in NHOK, HPV-immortalized HOK (HOK-16B, HOK-18A, and HOK-18C), and SCC-4 cells*.

Host Cells

Concentration of

MNNG(mg/ml)

Plasmid Recovered

# of Mutants/# of Total Colonies

Mutagenesis

Frequency (%)

NHOK
0 8/16,785 0.047

NHOK
0.5 14/14,750 0.095

NHOK
1.5 27/15,048 0.179

HOK-16B
0 18/16,872 0.107†

HOK-16B
0.5 71/20,534 0.346††

HOK-16B
1.5 85/15,187 0.560†††

HOK-18A
0 40/22,538 0.178†

HOK-18A
0.5 121/21,080 0.574††

HOK-18A
1.5 146/16,631 0.878†††

HOK-18C
0 23/15,880 0.150†

HOK-18C
0.5 102/18,078 0.546††

HOK-18C
1.5 111/15,080 0.736†††

SCC-4
0 112/18,003 0.622†

SCC-4
0.5 134/18,930 0.708††

SCC-4
1.5 140/15,098 0.927†††

 

†Significantly higher than the mutation frequency in unexposed NHOK (Fisher’s exact test, double tail).

††Significantly higher than the mutation frequency in NHOK exposed to MNNG (0.5 m g/ml) (Fisher’s exact test, double tail).

†††Significantly higher than the mutation frequency in NHOK exposed to MNNG (1.5 m g/ml) (Fisher’s exact test, double tail).

*published in Shin et al., 1996.