Electronic Journal of Biotechnology ISSN: 0717-3458
  © 1999 by Universidad Católica de Valparaíso -- Chile
Vol. 2 No. 3, Issue of December 15, 1999
 

 

Figure 3. Schematic representation of the PCR strategy used to clone an apple pgip gene.

A. pIPGIP which contains the ipgip fragment used to design InvPCR primers AP-PGIP-INVL (INVL) and AP-PGIP-INVR (INVR).

B. BglII InvPCR clone, pAppBglII. Location of the primers used for InvPCR (INVL and INVR) is indicated. The 98bp and 166bp overlaps between the BglII InvPCR clone and the ipgip fragment are shown by solid blue boxes.

C. NsiI InvPCR clone. The positions of the primers AP-PGIP-INVL-2 (INVL-2) and AP-PGIP-INVR-2 (INVR-2), designed to sequences from pAppBglII, are indicated. The 190bp overlap between pAppBglII and pAppNsiI is indicated by a solid red box.

D. The composite apple pgip gene constructed using sequences obtained from pIPGIP, pAppBglII and pAppNsiI. B, BglII; E, EcoRV; H, HindIII; N, NsiI; X, XbaI.

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