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Electronic Journal of Biotechnology ISSN: 0717-3458
  © 2011 by Universidad Católica de Valparaíso -- Chile
Vol. 14 No. 3, Issue of May 15, 2011
 

Table 1. Fingerprinting performed on whole-genome amplification (WGA) of small Rotylenchulus reniformis samples and on their original population. GenomiPhi and GenomePlex are commercial kits used for WGA. §: 1 and 5 refer to the number of gravid females with their associated egg masses used for WGA.



-

Whole Genome Amplification

-
-

GenomiPhi

GenomePlex

MSRR03 population 10,000 eggs

-

1§

5

1

5

 Markers with amplification
 (total 96 SSR markers  tested)

55

58

57

62

71

 Total number of alleles
 observed on 96 SSR markers
 tested

76

87

86

102

109

 Number of alleles detected
 that were also present in the
 MSRR03 population

71
(93%)

77
(88%)

74
(86%)

89
(87%)

 

 Average number of
 alleles/marker

1.36

1.45

1.48

1.57

1.54

 Standard deviation of number
 of alleles/marker

0.59

0.72

0.75

0.9

0.69

 Min-max number of alleles per
 marker

1-3

1-4

1-4

1-5

1-4

 Number of SSR markers with
 100% of alleles also present
 in the MSRR03 population

51
(93%)

52
(90%)

50
(88%)

53
(85%)