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Electronic Journal of Biotechnology ISSN: 0717-3458
  © 2011 by Universidad Católica de Valparaíso -- Chile
Vol. 14 No. 3, Issue of May 15, 2011
 

Table 5. The effective number of patterns and discriminatory power obtained for assay unit for Brassica oleracea var. capitata AFLP.



multiplex

primer combinations

Ns = 20

MseI

EcoR1

I

I/Ns

P

%P

Pu

Pu/Ns

Dj

1

CAT

ACAFAM

18

0.9

15,4

85.5

 15,1

 0.757

0.9842

CAT

AGGJOE

19

1.0

18,2

95.7

0.9947

CAT

ACCNED

16

0.8

11,8

73.5

0.9632

2

CTT

ACAFAM

17

0.9

15,4

90.5

9

 0.448

0.9842

CTT

AGGJOE

12

0.6

8,7

72.4

0.9275

CTT

ACCNED

6

0.3

2,8

46.8

0.6743

3

CAC

ACTFAM

20

1.0

20,0

100.0

17,5

 

0.875

 

1.0000

CAC

AAGJOE

17

0.9

12,5

73.5

0.9684

CAC

AACNED

20

1.0

20,0

100.0

1.0000

4

CAG

ACTFAM

16

0.8

13,3

83.3

 

17,8

 

 

0.888

 

0.9737

CAG

AAGJOE

20

1.0

20,0

100.0

1.0000

CAG

AACNED

20

1.0

20,0

100.0

1.0000

5

CTC

ACTFAM

17

0.9

15,4

90.5

 

18,5

 

 

0.923

 

0.9842

CTC

AAGJOE

20

1.0

20,0

100.0

1.0000

CTC

AACNED

20

1.0

20.0

100.0

1.0000

6

CTG

ACTFAM

15

0.8

10,5

70.2

12,2

0.612

0.9526

CTG

AAGJOE

18

0.9

16,7

92.6

0.9895

CTG

AACNED

13

0.7

9,5

73.3

0.9421

7

CAT

ACGJOE

11

0.6

6,7

60.6

12,6

0.630

0.8947

CAT

AGCNED

19

1.0

18,5

97.3

0.9952

8

CTT

ACGJOE

17

0.9

15,4

90.5

17,7

0.885

0.9842

CTT

AGCNED

20

1.0

20,0

100.0

1.0000

9

CAC

ACGJOE

16

0.8

14,5

90.5

16,4

0.818

0.9794

CAC

AGCNED

19

1.0

18,2

95.7

0.9947

10

CAG

ACGJOE

18

0.9

15,4

85.5

17,7

0.885

0.9842

CAG

AGCNED

20

1.0

20,0

100.0

1.0000

11

CTC

ACGJOE

20

1.0

20.0

100.0

20.0

1.000

1.0000

CTC

AGCNED

20

1.0

20.0

100.0

1.0000

12

CTG

ACGJOE

9

0.5

5,8

64.4

7,7

0.383

0.8632

CTG

AGCNED

12

0.6

9,5

79.4

0.9421

Ns = number of samples, I = number of distinct patterns, P = effective number of patterns per assay unit, % P = percentage of a effective number of patterns from the number of patterns, Pu = average effective number of patterns per multiplex, Dj = discriminatory power. FAM, JOE, NED = blue, green, yellow fluorescent dye.