EJBiotechnology logo
Electronic Journal of Biotechnology ISSN: 0717-3458
  © 2011 by Universidad Católica de Valparaíso -- Chile
Vol. 14 No. 3, Issue of May 15, 2011
 

Table 4. Summary of obtained AFLP fragments in Brassica oleracea var. capitata and Brassica pekinensis (22 accessions). Results within Brassica oleracea var. capitata (20 accessions) are noted separately.



multiplex

primer
combinations

B. oleracea var. capitata + B. pekinensis

B. oleracea var. capitata

No. of
frag.

No. of
polymorphic
frag.

Percentage of
polymorphic
frag.

No. of
frag.

No. of
polymorphic
frag.

Percentage of
polymorphic
frag.

MseI

EcoR1

 

CAT

ACAFAM

26

22

84,6

22

10

45,5

1

CAT

AGGJOE

24

20

83,3

20

14

70,0

 

CAT

ACCNED

24

22

91,7

20

9

45,0

 

CTT

ACAFAM

40

35

87,5

25

11

44,0

2

CTT

AGGJOE

26

20

76,9

18

6

33,3

 

CTT

ACCNED

23

21

91,3

16

5

31,3

 

CAC

ACTFAM

39

37

94,9

22

13

59,1

3

CAC

AAGJOE

40

37

92,5

30

12

40,0

 

CAC

AACNED

43

39

90,7

30

9

30,0

 

CAG

ACTFAM

25

23

92,0

17

7

41,2

4

CAG

AAGJOE

55

51

92,7

38

19

50,0

 

CAG

AACNED

41

39

95,1

30

19

63,3

 

CTC

ACTFAM

27

25

92,6

21

9

42,9

5

CTC

AAGJOE

36

32

88,9

30

19

63,3

 

CTC

AACNED

48

41

85,4

33

15

45,5

 

CTG

ACTFAM

28

25

89,3

23

12

52,2

6

CTG

AAGJOE

33

31

93,9

26

11

42,3

 

CTG

AACNED

34

25

73,5

25

6

24,0

7

CAT

ACGJOE

19

15

78,9

12

6

50,0

 

CAT

AGCNED

67

51

76,1

57

25

43,9

8

CTT

ACGJOE

30

25

83,3

17

7

41,2

 

CTT

AGCNED

61

56

91,8

52

29

55,8

9

CAC

ACGJOE

31

27

87,1

27

10

37,0

 

CAC

AGCNED

49

41

83,7

38

12

31,6

10

CAG

ACGJOE

31

24

77,4

26

8

30,8

 

CAG

AGCNED

53

49

92,5

33

18

54,5

11

CTC

ACGJOE

37

31

83,8

28

16

57,1

 

CTC

AGCNED

47

38

80,9

37

16

43,2

12

CTG

ACGJOE

17

15

88,2

12

5

41,7

 

CTG

AGCNED

30

26

86,7

21

7

33,3

Total no. of peaks

1084

943

87,0

806

365

45,3

Mean no. of peaks per combination

36,1

31,4

 

26,9

12,2

 

frag. = AFLP fragments; FAM, JOE, NED = blue, green, yellow fluorescent dyes.