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Electronic Journal of Biotechnology ISSN: 0717-3458
  © 2011 by Universidad Católica de Valparaíso -- Chile
Vol. 14 No. 3, Issue of May 15, 2011
 

Table 3. Influence of enzyme/albumin ratio, glutaraldehyde concentration, and pH on the size and hydrolytic activity of CLEAs.



CLEA2

Hydrolysis
activity (U/g)

Particle size
(mm)

pH of
glutaraldehyde

concentration
(g glutaraldehyde /l)

Enzyme/albumin
ratio (mg/mg)

4.5

1.5

5

2034 ± 17

25.8 ± 8.7

4.5

1.5

15

2874 ± 20

27.1 ± 7.9

4.5

3.5

5

1272 ± 19

26.9 ± 9.4

4.5

3.5

15

2670 ± 18

21.2 ± 5.1

9.5

1.5

5

894 ± 16

33.3 ± 9.4

9.5

1.5

15

1578 ± 17

57.9 ± 23.8

9.5

3.5

5

882 ± 17

83.4 ± 24.9

9.5

3.5

15

2214 ± 19

57.2 ± 21

2Each individual CLEA was done twice and each measurement was done in triplicate.