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Electronic Journal of Biotechnology ISSN: 0717-3458
  © 2011 by Universidad Católica de Valparaíso -- Chile
Vol. 14 No. 3, Issue of May 15, 2011
 

Table 1. Residual activities of CLEAs prepared with dextran aldehyde and glutaraldehyde at pH 4.5 and 9.5, after 50 hrs in buffer phosphate 25 mM at pH 7.0 and 60ºC. The percentages are referred to the initial suspension activity (100%).



 

CLEA1

Suspension
activity (%)2

Residual activity
in CLEAs (%)

Cross-linking reagent

Concentration
(g cross-linking reagent /l)

Enzyme/albumin ratio (mg/mg)

Dextran aldehyde

1.7

5

121.5 ± 1.7

45.7 ± 0.7

1.7

15

98.6 ± 1.1

46.8 ± 0.6

2.2

5

82.9 ± 0.9

21 ± 0.6

2.2

15

109.1 ± 1.3

34.1 ± 0.4

2.7

5

72.5 ± 0.9

26.4 ± 0.5

2.7

15

56.1 ± 1

5.5 ± 0.3

Glutaraldehyde pH 4.5

1.5

5

65.8 ± 0.9

37.8 ± 0.3

1.5

15

40.9 ± 1.1

20 ± 0.4

3.5

5

48.6 ± 1.2

2.8 ± 0.4

3.5

15

116 ± 1.3

69.7 ± 0.7

Glutaraldehyde pH 9.5

1.5

5

61.7 ± 1.1

40.9 ± 0.6

1.5

15

65 ± 1.4

32.7 ± 0.7

3.5

5

65.3 ± 1.1

51 ± 0.7

3.5

15

93.2 ± 1.5

70.2 ± 0.8

1Each individual CLEA was done twice and each measurement was done in duplicate.
2The absolute values corresponding to 100% are shown on Table 2 and Table 3 for each CLEA as Hydrolysis activity (U/g).