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Electronic Journal of Biotechnology ISSN: 0717-3458
  © 2011 by Universidad Católica de Valparaíso -- Chile
Vol. 14 No. 3, Issue of May 15, 2011
 


Fig. 3 Amplification of hLIF coding sequence by RT-PCR. The coding sequence of hLIF gene was successfully reverse transcribed and amplified, which is 609 bp (lanes 1), and lanes 2 was the full-length hLIF by exon-splicing. The figure indicated that it is more easily to obtain the coding sequence of target gene by exon-splicing in terms of quantity, compared with the traditional reverse transcription. "M" is DNA marker I.