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Electronic Journal of Biotechnology ISSN: 0717-3458
  © 2011 by Universidad Católica de Valparaíso -- Chile
Vol. 14 No. 3, Issue of May 15, 2011
 


Fig. 1 Amplification and splicing of hLIF coding sequence by exon-splicing. The exons were amplified by the standard PCR with the exons own primers, and that hLIF was performed by the overlapping PCR with P1 and P4. The length of the performed fragments of exon2, exon3 and the full-length hLIF is 213 bp, 426 bp, and 609 bp respectively. M is DNA marker I.