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Electronic Journal of Biotechnology ISSN: 0717-3458
  © 2011 by Universidad Católica de Valparaíso -- Chile
Vol. 14 No. 3, Issue of May 15, 2011

Fig. 3 Western blots of (a) JNK1, (b) p-JNK1, (c) c-Jun, (d) pc-Jun, (e) β-actin. This is a representation of three separate experiments. Rapamycin ‘-’ indicates no pre-treatment and ‘+’ indicates 2 hrs of rapamycin pre-treatment. TNF-α ‘-’ signifies unstimulated samples and ‘+’ signifies 2 hrs of TNF-α stimulation. * significant increase at p < 0.05 vs control. # significant decrease at p < 0.05 vs control. Treatment with rapamycin abrogated JNK1 activation. Rapamycin and TNF-α both activate c-Jun singly, but treatment with TNF-α reduces c-Jun activation after rapamycin treatment. β-actin was used as loading control. Densities for bands were recorded beneath each blot and were normalised to their respective loading controls.