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Electronic Journal of Biotechnology ISSN: 0717-3458
  © 2011 by Universidad Católica de Valparaíso -- Chile
Vol. 14 No. 3, Issue of May 15, 2011
 


Fig. 2 Expression of the GST fused rSLLyz. (a) Expression pattern analysis of the GST fused rSLLyz at different growth conditions. M, protein marker; lane 1, the GST fused rSLLyz was expressed at OD600 = 0.3 with 0.1 mM IPTG for 2 hrs; lane 2, OD600 = 0.3 with 0.1 mM IPTG for 3 hrs; lane 3, OD600 = 0.3 with 0.3 mM IPTG for 2 hrs; lane 4, OD600 = 0.3 with 0.3 mM IPTG for 3 hrs; lane 5, OD600 = 0.5 with 0.1 mM IPTG for 2 hrs; lane 6, OD600 = 0.5 with 0.1 mM IPTG for 3 hrs; lane 7, OD600 = 0.5 with 0.3 mM IPTG for 2 hrs; lane 8, OD600 = 0.5 with 0.3 mM IPTG for 3 hrs; lane 9, GST. (b) SDS-PAGE analysis of solubilized and thrombin treated inclusion bodies. M, protein marker; lane 1, solubilized inclusion body with 8 M urea; lane 2, thrombin cleavage with 5 mM β-mercaptoethanol; lane 3, thrombin cleavage with 1 mM β-mercaptoethanol; lane 4, GST.