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Electronic Journal of Biotechnology ISSN: 0717-3458
  © 2011 by Universidad Católica de Valparaíso -- Chile
Vol. 14 No. 3, Issue of May 15, 2011
 


Fig. 1 Cloning strategy of the recombinant S. litura lysozyme (rSLLyz) gene into pGEX-4T-1 vector. (a) Schematic diagram of the construction of expression vectors for the GST fused rSLLyz. (b) Structure of rSLLyz gene. Mature region of SLLyz gene was amplified with primers SLLyz EcoRI and SLLyz XhoI. The amplified lysozyme gene fragment and pGEX-4T-1 expression vector were digested with both EcoRI and XhoI, ligated to produce the GST fused rSLLyz, and transformed into Rosetta(DE3) Singles for recombinant lysozyme expression.