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Electronic Journal of Biotechnology ISSN: 0717-3458
  © 2011 by Universidad Católica de Valparaíso -- Chile
Vol. 14 No. 3, Issue of May 15, 2011
 

Table 1. In vitro development of bovine NT embryos derived from 3 different fetal sources and from 2 transfected cell lines derived from 1 fetus.



Cell Line

Nº of embryos cultured

Cleavage
n (%)

Blastocyst
n (%)

number of cells
(mean ± SD)

BFF 1

118

76 (64)

25 (21)a

95 ± 9.1

BFF 2

119

72 (61)

21 (18)a

81 ± 11.1

BFF 3

119

70 (59)

13 (11)b

84 ± 5.8

BFF tg 1.1

115

73 (64)

20 (18)a

85 ± 12.0

BFF tg 1.2

119

72 (61)

12 (10)b

83 ± 14.5

BFF: Non-transfected bovine fetal fibroblast cell lines derived from different fetuses. BFF tg: Transfected bovine fetal fibroblast clonal cell lines derived from fetus 1. Experiments represent 4 replicates. Cleavage, blastocyst and nuclei stain were registered at 72, 168 and 168 hrs, respectively. Data followed by different letters are significantly different (P < 0.05).