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Electronic Journal of Biotechnology ISSN: 0717-3458
  © 2011 by Universidad Católica de Valparaíso -- Chile
Vol. 14 No. 2, Issue of April 15, 2011
 

Table 2. Lactate dehydrogenase (LDH) release in KB cells untreated and treated with jasmonic acid (JA) and compounds 1-3 at different concentrations for 72 hrs. Stock solution of compounds was prepared in DMSO and the final concentration of this solvent was kept constant at 0.25%. Control cultures received DMSO alone.



Treatments

KB cells
% LDH released

Control

4.2 ± 0.5

Compound JA
12.5 µM
25 µM

4.9 ± 0.3
7.1 ± 0.7

Compound 1
12.5 µM
25 µM

6.3 ± 0.7
6.6 ± 0.3

Compound 2
12.5 µM
25 µM

6.2 ± 0.4
3.9 ± 0.7

Compound 3
12.5 µM
25 µM

5.5 ± 0.6
4.3 ± 0.2

The results are expressed as percentage of LDH released into the cell medium with respect to total LDH. Each value represents the mean ± SD of three experiments, performed in quadruplicate.