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Electronic Journal of Biotechnology ISSN: 0717-3458
  © 2010 by Universidad Católica de Valparaíso -- Chile
Vol. 13 No. 5, Issue of September 15, 2010
 


Figure 1. Qualitative and quantitative estimation of RNA isolated by present and others methods.
(a) Total RNA isolated using present protocol from different internodal tissues of Bambusa balcooa. RNA (approximately 1.0 µg each) from 2nd; 5th and 10th in Lanes 1, 2 and 3 respectively were electrophorosed on 1.2% (w/v) formaldehyde denaturing agarose gel. The 28S and 18S rRNA bands were indicated by arrows.
(a1) Electrophoresis of PCR amplified GAPDH gene on 1% agarose gel using isolated RNA by present protocol as template (Lane 1); cDNA prepared from isolated RNA as template (Lane 2) and genomic DNA of bamboo as template (Lane 3).
(b) Comparison of total RNA isolated from 5th internode of Bambusa balcooa by different method on 1.2% (w/v) formaldehyde denaturing agarose gel. Lanes 1, using present method; 2, using method of Chan et al. 2007; 3, using method of Vasanthaiah et al. 2008; 4, using method of Chiu et al. 2006; 5, by Trizol Reagent (Invitrogene, cat.# 10296010); 6, using method of Weng et al. 2009.
(b1) RT-PCR band (GADPH, 476 bp) obtained using RNA isolated from 5th internode by different protocols. Lanes 1, using present method; 2, using method of Chan et al. 2007; 3, using method of Vasanthaiah et al. 2008; 4, using method of Chiu et al. 2006; 5, from Trizol Reagent; and 6, using method of Weng et al. 2009.
(b2) The GADPH gene was detected on northern blot using total RNA isolated from 5th internodal bamboo tissue by different methods. Lanes 1, using present method; 2, using method of Chan et al. 2007; 3, using method of Vasanthaiah et al. 2008; 4, using method of Chiu et al. 2006; 5, by Trizol reagent; and 6, using method of Weng et al. 2009.
(c) A representation of subtraction cDNA library constructed using RNA from 2nd and 10th internode of bamboo. PCR amplifications of inserts obtained from different subtractive cDNA clones using SP6 and T7 primers. PCR products were run with a 100 bp marker (Bangalore Genei, cat.#105993) in a 1.0% agarose gel.


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