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Electronic Journal of Biotechnology ISSN: 0717-3458
  © 2010 by Universidad Católica de Valparaíso -- Chile
Vol. 13 No. 5, Issue of September 15, 2010
 

Table 2. Thirty G. arboreum ESTs showing amplified size of the amplicons, the number of SNPs detected for each EST, and their sequence and position in the amplicon.



Sr #

Best Blast homology

Conserved region

Amplicons size

No. of SNPs/
Indels

SNP/ Indel location/ position

1

 Histone H2B-3

350-650

300

2

+A  (595)
- C  (634)

2

 NADPH-cytochrome P450  reductase

450-550

100

0

-

3

 Werewolf

201-570

400

1

- G (562)

4

 putative ATP binding protein

30-150

120

2

T→C (30)
A→T (32)

5

 common regulatory factor

262-562

300

1

A→G (558)

6

 Photolyase/blue light  photoreceptor PHR2 

20-200

180

0

-

7

 S-adenosyl-L-homocysteine  hydrolase

246-663

420

3

C→A (249)
C→T (412)
C→A (646)

8

 Mitochondrial small heat shock  protein

139-440

300

0

-

9

 S-adenosyl methionine  synthetase

193-332

120

1

G→A (229)

10

 Histone H2B1 

190-500

310

0

-

11

 Actin-depolymerizing factor 2

300-500

200

0

-

12

 CONSTANS-like protein 1

162-440

280

1

+A (178)

13

 Profilin

35-540

500

0

-

14

 60S ribosomal protein L24

134-441

300

1

C→G (89)

15

 Similar to ATP-citrate-lyase

79-424

350

0

-

16

 Contains similarity to protein  kinase~gene

10-450

440

1

-A (12)

17

 putative transcription factor  BHLH6

111-296

200

1

-T (159)

18

 60S ribosomal protein L4/L1

169-346

200

0

-

19

 Histone H3.3

169-392

225

0

0

20

 Luminal binding protein  (BiP)

46-411

365

2

A→C (49)
C→T (50)

21

 Cystein proteinase

267-356

100

0

0

22

 Outer membrane lipoprotein –  like

40-428

400

0

0

23

 14-3-3-like protein

275-560

300

0

-

24

 Nucleotide-binding protein

148-230

200

1

G→C (204)

25

 RAD23 protein

23-105

80

0

-

26

 Elongation factor 1-gamma

848-1010

160

3

G→C (899)
A→G (920)
A→G (947)

27

 E2, Ubiquitin-conjugating  enzyme, putative

10-378

370

0

-

28

 Beta-galactosidase-1

151-380

240

2

C→A (379)
T→A (380)

29

 Alcohol dehydrogenase

299-476

200

2

G→ T (431)
A→G (462)

30

 vacuolar H+-ATPase subunit E

70-214

144

3

G→A (137)
G→A (153)
A→G (217)

 


Supported by UNESCO / MIRCEN network