Figure 2. Expression of the PyGUS reporter gene in gametophytes and protoplasts prepared from allantoin-treated
and b) GUS expression of control and 10 mM allantoin-treated gametophytic
blades, respectively, for which the number of PyGUS expressing cells was equal.
(c-m) Normal regeneration of transiently transformed protoplasts in which the PyGUS gene was overexpressed under the direction of the PyAct1 promoter. PyGUS
expression was observed in protoplast prepared from particle-bomberded
gametophytic blades (c) and during regeneration of these protoplasts (d-m).
bars: a and b, 100 µm; c-m,