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Electronic Journal of Biotechnology ISSN: 0717-3458
  © 2010 by Universidad Católica de Valparaíso -- Chile
Vol. 13 No. 2, Issue of March 15, 2010

Figure 1. Comparison of the ability in regeneration of P. yezoensis protoplasts prepared by the treatment with AMX solution and allantoin. Scale bars: 20 µm.
(a) Protoplast prepared from gametophytic blades by treatment with AMX solution. No regeneration was observed.
(b) Protoplast prepared from gametophytic blades which were cultured in the ESL medium containing 10 mM allantoin for 3 weeks.
(c) Ability of regeneration in protoplasts prepared from allantoin-treated gametophytic blade.

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