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Electronic Journal of Biotechnology ISSN: 0717-3458
  © 2010 by Universidad Católica de Valparaíso -- Chile
Vol. 13 No. 2, Issue of March 15, 2010
 


Table 1. Parameters evaluated for the adaptation of AFLPs® in C. vulgaris.



Step

Parameter

variants

 A
 (Restriction digestion)

 Duration

3

 Temperature

4

 Buffer system

3

 Enzyme distributor

3

 Enzyme proportions

20

 B
 (Ligation)

 Duration

3

 Temperature

3

 Buffer system

3

 Enzyme distributor

3

 C
 (PCR amplification)

 Enzyme distributor

3

 distributor of IRDye oligos

3

 Oligo proportions

5

 Dilutions

5

 D
 (Separation and
 Visualization)

 Gel matrix solution

2

 Loading dye

2

 Buffer composition

2

 

Supported by UNESCO / MIRCEN network