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Electronic Journal of Biotechnology ISSN: 0717-3458
  © 2010 by Universidad Católica de Valparaíso -- Chile
Vol. 13 No. 1, Issue of January 15, 2010
 


Figure 3. Results of gel-shift assay for the AtGST11 promoter region. (a) Series of DNA probes used for this gel-shift assay. Approximately the 1677 bp upstream region and the 80 bp of open reading frame of the AtGST11 gene was divided into the eight overlapped DNA segments (Fragment 1; -1677~-1394 bp Fragment 2, -1467~-1193 bp; Fragment 3, -1288~-1004 bp; Fragment 4, -1079~-806 bp; Fragment 5, -895~-611 bp; Fragment 6, -683~-397 bp; Fragment 7, -463~-189 bp; Fragment 8, -234~+80 bp) and then labeled with Dig-11-dd-UTP at the 3’ terminal end. These Dig-labeled DNA probes were mixed with isolated #13 or #43 proteins and then applied to polyacrylamide gel electrophoresis. (b) Result of gel-shift assay for #13 (Left) and #43 (Right). Lane numbers showed the DNA fragment number used for the binding reaction.


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