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Electronic Journal of Biotechnology ISSN: 0717-3458
  © 2010 by Universidad Católica de Valparaíso -- Chile
Vol. 13 No. 1, Issue of January 15, 2010
 


Table 3. The effect of BAP and AS on the number of shoots induced from embryogenic axes of Phaseolus vulgaris L. after 30 days of culture on induction medium supplemented with different BAP and AS concentrations.


Treatments

Shoots1 (efficiency %)2

BAP (mgl-1)

AS (mgl-1)

Bribrí

Brunca

Guaymí

Huetar

Telire

 0

 0

 0 ± 03 c (0)

 0 ± 0 c (0)

 0 ± 0 a (0)

 0 ± 0 a (0)

 0 ± 0 c (0)

 0

 20

 0 ± 0 c (0)

 0 ± 0 c (0)

 0 ± 0 a (0)

 0 ± 0 a (0)

 0 ± 0 c (0)

 0

 40

 0.03 ± 0.03 c (3)

 0.93 ± 0.05 b (93)

 0 ± 0 a (0)

 0.10 ± 0.06 a (10)

 0.80 ± 0.07 ab (80)

 5

 0

 0 ± 0 c (0)

 0.47 ± 0.18 b c (21)

 0 ± 0 a (0)

 0 ± 0 a (0)

 0 ± 0 c (0)

 5

 20

 1.20 ± 0.22 a (53)

 0.53 ± 0.20 b (24)

 0.13 ± 0.06 a (10)

 0 ± 0 a (0)

 0.93 ± 0.15 a (67)

 5

 40

 0.17 ± 0.07 bc (17)

 2.43 ± 0.17 a (96)

 0.17 ± 0.07 a (17)

 0.07 ± 0.05 a (7)

 0.45 ± 0.11 abc (60)

 10

 0

 0.07 ± 0.07 bc (7)

 0 ± 0 c (0)

 0 ± 0 a (0)

 0 ± 0 a (0)

 0 ± 0 c (0)

 10

 20

 0.50 ± 0.17 b (20)

 0 ± 0 c (0)

 0.13 ± 0.06 a (13)

 0 ± 0 a (0)

 0.37 ± 0.25 bc (10)

 10

 40

 0.20 ± 0.07 bc (20)

 0.77 ± 0.14 b (57)

 0.13 ± 0.06 a (13)

 0 ± 0 a (0)

 0.90 ± 0.13 a (70)

1 Values represent the average shoot formation in three Petri dishes with 10 explants and three replicates.
2 [(Number of embryogenic axes with novo shoots/total of embryogenic axes) x 100]
3 Mean ± SE
Same letters within columns denote statistically equal means with the Tukey Unequal N HSD test (P < 0.05). Treatments that gave the best results of shoot formation are highlighted in bold.


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