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Electronic Journal of Biotechnology ISSN: 0717-3458
  © 2010 by Universidad Católica de Valparaíso -- Chile
Vol. 13 No. 1, Issue of January 15, 2010
 


Table 2. Effect of BAP and AS concentration on phenolic oxidation and average number of shoots, roots and leaves induced from embryogenic axesofcommon bean (Phaseolus vulgaris L.) regardless of the genotype. Data were recorded after 30 days of culture on induction medium supplemented with different BAP and AS concentrations.


Treatments

  

  

  

  

BAP (mgl-1)

AS (mgl-1)

Shoots1

Leaves1

Roots1

Phenolic oxidation1

 0

 0

 0.00 ± 0.002 d

 0.89 ± 0.05 a

 3.53 ± 0.18 a

 0.00 ± 0.00 e

 0

 20 

 0.00 ± 0.00 d

 0.34 ± 0.04 d

 1.85 ± 0.16 b

 0.19 ± 0.03 c d

 0

 40

 0.37 ± 0.04 b c

 0.81 ± 0.07 a b

 0.65 ± 0.05 c

 0.45 ± 0.04 a

 5

 0

 0.09 ± 0.04 d

 0.00 ± 0.00 e

 0.09 ± 0.04 d

 0.00 ± 0.00 e

 5

 20

 0.56 ± 0.08 a b

 0.70 ± 0.13 a b c

 0.01 ± 0.01 d

 0.31 ± 0.04 b c

 5

 40

 0.66 ± 0.09 a

 0.49 ± 0.09 c d

 0.00 ± 0.00 d

 0.05 ± 0.02 d e

 10

 0

 0.01 ± 0.01 d

 0.00 ± 0.00 e

 0.00 ± 0.00 d

 0.31 ± 0.04 b c

 10

 20

 0.20 ± 0.06 c d

 0.25 ± 0.06 d e

 0.31 ± 0.08 c d

 0.43 ± 0.04 a b

 10

 40

 0.40 ± 0.05 b c

 0.51 ± 0.08 b c d

 0.00 ± 0.00 d

 0.15 ± 0.02 d

1 Values represent the average shoot, leaves and roots formation per treatment in 15 Petri dishes with 10 explants and three replicates.
2 Mean ± SE
Same letters within columns denote statistically equal means with the Tukey Unequal N HSD test (P< 0.05).


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