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Electronic Journal of Biotechnology ISSN: 0717-3458
  © 2010 by Universidad Católica de Valparaíso -- Chile
Vol. 13 No. 1, Issue of January 15, 2010
 


Table 2. Initial reaction rates (v) for free (FPGA) and immobilized (SP1) penicillin acylase at different penicillin G concentrations at pH 7.8 and 30ºC, and calculated kinetic parameters. In the case of SP1, kinetic parameters are intrinsic.


FPGA

SP1

[penicillin G]
(mM)

v (mmol min-1 gprot-1)

[penicillin G]
(mM)

v (mmol/min·gprot)

0.08

18.7

 

 

0.1

21.2

 

 

0.14

24.4

0.14

1.82

 

 

0.17

1.96

 

 

0.20

2.24

0.23

29.3

0.23

2.67

 

 

0.3

3.65

0.5

35.0

0.5

5.75

 

 

0.8

8.41

 

 

2

14.78

 

 

4

16.51

10

41.6

10

17.40

 

 

25

17.73

KM (mM)

Vmax (mmol/min·gprot)

KM (mM)

Vmax (mmol/min·gprot)

0.10

42.0

0.45

18.18

 


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