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Electronic Journal of Biotechnology ISSN: 0717-3458
  © 2010 by Universidad Católica de Valparaíso -- Chile
Vol. 13 No. 1, Issue of January 15, 2010
 


Table 1. Protein immobilization yield of large and small particle (LP and SP) size Sepharose 6B-CL immobilized penicillin G acylase at different protein loadings.



Biocatalyst

Loaded protein
(g ggel-1)

Immobilized protein
(g ggel-1)

Protein immobilization
yield
(%)

LP1

0.016

0.0154

97

LP2

0.035

0.0341

98

LP3

0.054

0.053

98

LP4

0.073

0.0691

95

LP5

0.092

0.0824

90

LP6

0.13

0.0912

70

SP1

0.016

0.0154

97

SP2

0.035

0.0341

98

SP3

0.054

0.0529

98

SP4

0.073

0.0698

96

SP5

0.092

0.0843

92

SP6

0.13

0.0893

69

 


Supported by UNESCO / MIRCEN network