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Electronic Journal of Biotechnology ISSN: 0717-3458
  © 2010 by Universidad Católica de Valparaíso -- Chile
Vol. 13 No. 1, Issue of January 15, 2010
 


Table 1. Quantitative potency assay for pVGI.1(VEGF2) produced in different hosts and stability testing.



Experiment

Host

Treatment

DNA Concentration by A260 (mg/ml)

Potency Value Calculated by SoftMax Pro® (mg/ml)

Change in Response
Curve
(4 PL Parameters)

1

DH5a

Untreated

33

30

-

DH5a

Heat treated

(90ºC for 8 hrs)

33

9

Reduction in upper asymptote
(ratio of standard/sample changed from 1 to 1.3)

DH5a

Sheared

(22 gauge needle for 30 times)

33

11

Reduction in upper asymptote (ratio of standard/sample changed from 1 to 1.3))

2

DH5a

Untreated

1000

1000

-

Non-DH5a host

Untreated

1000

780

Change in parameter B
(change from 3.383 ± 1.066 to 17.521)


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