Figure 2. Qualitative cell proliferation assay.
(a) Endoethelial cell proliferation
induced by supernatants of host cells transfected with serially diluted pVGI.1(VEGF2) (Squares) or the empty vector pVGI.0 (diamonds).
Cell proliferation was detected by BrdU incorporation, followed by
quantification of BrdU in the fixed and denatured cells. Each point is an
average of 9 replicates. Error bars show the standard deviation of the 9 results.
(b) Quantification of the VEGF-2 protein
expressed by serially diluted pVGI.1(VEGF2). The VEGF-2 protein was quantified
by receptor binding assay, with purified VEGF-C protein (R and D systems) as
standard. Each point is an average of 3 replicate. Standard deviation of the
three is shown as the error bar.