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Electronic Journal of Biotechnology ISSN: 0717-3458
  © 2009 by Universidad Católica de Valparaíso -- Chile
Vol. 12 No. 4, Issue of December 15, 2009
 

Table 1. Regeneration of eleven Cyclamen-genotypes using protocol 1.



Genotype / Source of explants

Number of explants

Number of sterile explants

Start of differentiation after months

Explants with somatic embryo-like structures or shoots*

Number of plantlets after 7 months

 

 

 

 

%

total

 

C. africanum

 

 

 

 

 

 

Placenta

8

8

0

0

 

0

Peduncle

8

4

3

50.0

2

0

Leaf

8

7

0

0

 

0

Petiole

20

16

7

12.5*

2

2*

C. cilicium

 

 

 

 

 

 

Leaf

8

4

0

0

0

Petiole

20

20

6

15.0

3

1

C. coum

 

 

 

 

 

 

Placenta

36

24

3

4.2

1

8

Peduncle

36

20

0

0

 

0

Leaf

8

6

0

0

 

0

Petiole

28

19

6

15.8

3

3

C. coum ssp. coum

 

 

 

 

 

 

Leaf

20

7

0

0

0

Petiole

24

20

6

5.0

1

3

C. coum magenta

 

 

 

 

 

 

Placenta

20

20

3

30.0

6

249

Peduncle

20

12

3

25.0

2

200

Leaf

20

10

6

20.0

2

10

Petiole

24

20

3

10.0

2

3

C. hederifolium ssp confusum

 

 

 

 

 

 

Placenta

4

4

0

0

0

Peduncle

4

4

0

0

0

Leaf

4

4

6

25.0

1

1

Petiole

16

14

6

14.3

2

8

C. hederifolium cv. ‘Perlenteppich’

 

 

 

 

 

 

Placenta

20

11

0

0

 

0

Peduncle

20

8

0

0

 

0

Leaf

16

7

0

0

 

0

Petiole

20

16

3

12.5

2

2

C. hederifolium cv. ‘Rosenteppich’

 

 

 

 

 

 

Placenta

20

10

0

0

0

Peduncle

20

7

7

42.9

3

3

Leaf

16

12

0

0

 

0

Petiole

20

20

0

0

 

0

C. persicum

 

 

 

 

 

 

Placenta

20

16

3

12.5

2

10

Peduncle

20

4

3

50.0

2

8

Leaf

4

4

0

0

 

0

Petiole

16

16

0

0

 

0

C. persicum pink

 

 

 

 

 

 

Leaf

4

4

0

0

 

0

Petiole

16

15

0

0

 

0

C. purpurascens

 

 

 

 

 

 

Placenta

20

15

3

6.7

1

16

Peduncle

20

20

3*

20.0*

4

13*

Leaf

12

12

6*

25.0*

3

50*

Petiole

20

20

3*

25.0*

4

71*

* Regenerants were classified as shoot formation


Supported by UNESCO / MIRCEN network