Electronic Journal of Biotechnology ISSN: 0717-3458
  © 2009 by Universidad Católica de Valparaíso -- Chile
Vol. 12 No. 3, Issue of July 15, 2009
 


Table 1. Description of buffer systems in shake-flask cultures.



Components

Concentrations (mM) and pH tested
Buffer capacity
pKa

Citric acid + NaOH

50 mM, pH 4.0; 50 mM, pH 4.8; 100 mM, pH 4.8

2.15-6.51

3.13; 4.76; 6.40

Formic acid + NaOH

50 mM, pH 4.0; 50 mM, pH 4.8; 100 mM, p H4.8

2.6-4.8

3.75

Potassium hydrogen phthalate + NaOH

50 mM, pH 4.0; 50mM, pH 4.8; 100 mM, pH 4.8

4.10-5.9

2.95; 5.41

Succinic acid + NaOH

50 mM, pH 4.0; 50 mM, pH 4.8; 100 mM, pH 4.8

3.8-6.0

4.21; 5.64


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