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Electronic Journal of Biotechnology ISSN: 0717-3458
  © 2008 by Universidad Católica de Valparaíso -- Chile
Vol. 12 No. 2, Issue of April 15, 2009

Figure 3. 1% agarose electrophoresis gel of L10/L12 ribosomal protein PCR products. Lanes 7 -11-undiseased; lanes 2-6 -positive 716bp amplification from infected samples; lanes 1 and 14 1kb DNA ladder; lane 12 negative control; lane 13 positive control. The results show that plants from tissue culture supported nurseries were HLB-disease free, while plants from low altitude nurseries/fields (1000 m above sea level) and high altitude (2000 m) not supported by tissue culture were infected with the ‘Candidatus Liberibacter africanus’ giving a PCR fragment of 716 base pairs.

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