EJBiotechnology logo
Electronic Journal of Biotechnology ISSN: 0717-3458
  © 2008 by Universidad Católica de Valparaíso -- Chile
Vol. 12 No. 2, Issue of April 15, 2009
 

Table 1. Effect of added Fe3+ and Mo6+ on activities of nitrate and nitrite reductase assayed in subcellular fractions and whole cells of Paracoccus pantotrophus P16.


Batchb

Trace metal (µM)

Specific activity
[nmol (mg protein)-1 min-1]a

Specific activity
[
nmol (mg DCW)-1 min-1] a

Fe3+

Mo6+

Membrane-bound
nitrate reductase

Periplasmic nitrite
reductase

Nitrate
reductase

Nitrite
reductase

1

-

-

387 ± 60

223 ± 13

39 ± 4

20 ± 1

2

-

2.4

536 ± 6

187 ± 24

44 ± 4

18 ± 3

3

1.5

-

998 ± 28

373 ± 18

240 ± 30

162 ± 13

4

1.5

2.4

6,223 ± 502

344 ± 20

324 ± 21

148 ± 22

a Values represent mean ± S.D. of three replicates.
b Batches 1-4 were performed in the medium containing glucose as a carbon source and at the ratio of C/N = 1.15.


Supported by UNESCO / MIRCEN network