Figure 1. Somatic embryogenesis derived from scalps
of the banana CIEN-BTA-03.
(a) Temporary immersion system.
(b-c) Multiple meristems at the base of the shoots
after 4 months in (a).
(d-e) Multiple small meristems (1-3 mm, arrow) coming from (b) and after two months in ME25.
(f-g) Tissue features after 16 months in ME22 or
ME25; note the formation of multiple meristems of diverse sizes.
(h-i) Non-embryogenic calluses; nodular ones were
frequent in all explants whereas compact ones were rare after 5 months.
(j-k) Primary somatic embryos (SE) developed from
scalps from TI25, at 3.5 months.
(l-m) Embryogenic callus with numerous SE (arrow) on
its periphery, adequate for the establishment of ECS.
(n) Predominant embryonic cells aggregate in the ECS,
at two months.
(p) Cell aggregate consisting of parenchymatous cells
with starch granules, highly frequent in NECS, at two months.
(q) Non-embryogenic cells in NECS, at 16 days.
(r) ECS, at four months.
(s-u) SE in maturation-medium, at 20 days (s-t) and
30 days (u).
(v-z) SE in embryo-conversion medium, at one month.
(aa-ab) Regenerated plantlet in MS plus 400 mg L-1 of BA, at 21 days and 1.5 months, respectively, coming from (U).
(a) Bar = 45 mm.
(b, f, l, s, ab) Bar = 10 mm.
(c, h, aa)
Bar = 5 mm.
(d, i-k, m) Bar = 1 mm.
(e, g, t-u) Bar = 2 mm.
(n, p-q) Bar
= 100 µm.
(ñ-o) Bar = 50 µm.
(r) Bar = 22 mm.
(v-z) Bar = 2,5 mm.