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Electronic Journal of Biotechnology ISSN: 0717-3458
  © 2008 by Universidad Católica de Valparaíso -- Chile
Vol. 11 No. 5, Special Issue of December 15, 2008
 

Table 1. Evaluation of transgenic cell lines expressing the red fluorescent protein (HcRed).


Cell Lines

Level of HcRed
expression
in cells

Karyotyping
analysis

Morfology/Nº
Passages

Cloned
Blastocyst/
Fluorescence

clone 1

Medium

2n=60,XX

Normal/>10

Yes/Low

clone 2

Low

2n=60,XX

Normal/>10

Yes/Low

clone 3

N. Detec.

N.D.

N.D.

N.D.

clone 4

N. Detec.

N.D.

N.D.

N.D.

clone 5

N. Detec.

N.D.

N.D.

N.D.

clone 6

Low

2n=58,XX

Senescent/<5

No

clone 7

N. Detec.

N.D.

N.D.

N.D.

clone 8

Low

2n=60,XX

Normal/>10

Yes/N. Detec.

clone 9

High

2n=60,XX

Senescent/<5

No

clone 10

N. Detec.

N.D.

N.D.

N.D.

clone 11

N. Detec.

N.D.

N.D.

N.D.

clone 12

N. Detec.

N.D.

N.D.

N.D.

clone 13

N. Detec.

N.D.

N.D.

N.D.

clone 14

N. Detec.

N.D.

N.D.

N.D.

clone 15

N. Detec.

N.D.

N.D.

N.D.

    Clones selected for later analysis; N. Detec.: Non Detected; N.D.: Non Determined.


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