Electronic Journal of Biotechnology ISSN: 0717-3458
  © 2008 by Universidad Católica de Valparaíso -- Chile
Vol. 11 No. 5, Special Issue of December 15, 2008
 


Figure 1. Developmental assessment of transgenic cloned embryos generated with bovine embryonic fibroblasts (BEF) expressing HcRed.
a) Transfected BEF expressing HcRed (20x);
b) Expression of the red fluorescent protein in the cellular nuclei (40x);
c) Karyotyping analysis of metaphase spreads of transfected BEF, with sexual chromosomes highlighted (arrows);
d) Nuclear transfer procedure with cells expressing HcRed;
e-h) Developmental assessment of transgenic cloned embryo expressing the red fluorescent protein in the blastomeres (HcRed).


Supported by UNESCO / MIRCEN network