Electronic Journal of Biotechnology ISSN: 0717-3458
  © 2008 by Universidad Católica de Valparaíso -- Chile
Vol. 11 No. 4, Issue of October 15, 2008
 


Figure 4. Analysis of recombinant Liv-MIH-1 protein expression in P. pastoris by Western blot. M: MultiMark Multicolored Standard (Invitrogen). Lanes 1 and 2 correspond to cultures induced with 2% MeOH: Lane 1, KM71; Lane 2, KM71 + pPICZαA without insert. Lane 3 corresponds to non-induced Liv-MIH-1. Lanes 4 and 5 correspond to Liv-MIH-1 induced with 0.5% MeOH: Lane 4, day 1; Lane 5, day 2. Lanes 6 and 7 correspond to Liv-MIH-1 induced with 2% MeOH: Lane 6, day 1; Lane 7, day 2. Lanes 8 and 9 correspond to Liv-MIH-1 induced with 4% MeOH: Lane 8, day 1; Lane 9, day 2.


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