Electronic Journal of Biotechnology ISSN: 0717-3458
  © 2008 by Universidad Católica de Valparaíso -- Chile
Vol. 11 No. 3, Issue of July 15, 2008
 

Table 3. A comparison of mRNA recovered and number of recombinant colonies obtained from Xylem tissue of Zinfandel (PD-Susceptible) and Blanc du Bois (PD-Tolerant) infected with Pierces Disease (PD) between original and modified protocol through subtractive hybridization.


Protocol

Xylem Tissue

Zinfandel (Bunch type)

Blanc du Bois (Florida hybrid)

mRNA
(μg/100 μg of total RNA)

No. of recombinant colonies obtained

mRNA
(μg/100 μg of total RNA)

No. of recombinant colonies obtained

Modified method

3.6

180

3.2

140

Salzman method

1.4

40

1.0

35


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