Electronic Journal of Biotechnology ISSN: 0717-3458
  © 2008 by Universidad Católica de Valparaíso -- Chile
Vol. 11 No. 3, Issue of July 15, 2008
 

Table 2. Yield and quality* of total RNA obtained from different grapevine tissue and species.


Species and cultivar

Grapevine Tissue

Absorbance ratios

Yield
(μg/g FW)

A260/A280

A260/A230

Vitis vinifera
cv. Zinfandel
(Bunch type)

Leaf

 Young
 Mature healthy
 Infected

1.90
1.67
1.87

2.12
2.22
2.14

540
384
272

Xylem

 Young
 Mature healthy
 Infected

1.87
1.70
1.54

2.36
2.11
2.24

520
347
284

Phloem

 Young
 Mature healthy
 Infected

1.84
1.76
1.52

2.10
2.32
2.22

287
246
132

Petiole

 Young
 Mature healthy
 Infected

1.64
1.55
1.54

2.16
2.24
2.36

460
244
178

Vitis spp cv.Lake Emerald
(Florida Hybrid)

Leaf

 Young
 Mature healthy
 Infected

1.87
1.72
1.64

2.34
2.24
2.11

276
214
110

Vitis rotundifolia cv. Carlos
(Muscadine)

Leaf

 Young
 Mature healthy
 Infected

1.90
1.86
1.68

2.28
2.14
2.10

532
480
275

Xylem

 Young
 Mature healthy
 Infected

1.88
1.62
1.54

2.26
2.13
2.11

209
178
112

Phloem

 Young
 Mature healthy
 Infected

1.72
1.66
1.61

2.31
2.29
2.10

184
096
054

Petiole

 Young
 Mature healthy
 Infected

1.82
1.75
1.72

2.27
2.18
2.14

140
084
032

*Results are expressed as the mean of 3 samples.

  Supported by UNESCO / MIRCEN network