Electronic Journal of Biotechnology ISSN: 0717-3458
  © 2008 by Universidad Católica de Valparaíso -- Chile
Vol. 11 No. 2, Issue of April 15, 2008

Figure 5. Purification of doublet by RP-HPLC.
(a) RP-HPLC elution profile of the 80% ACN post Sep-Pak C-18 with a gradient of 5-60% ACN.
(b) Protein profile resolved through Tris-tricine / Urea (18%) gel electrophoresis Lane 1: Molecular Weight Standards (Bio-Rad, CA, USA), Lane 2 eluate from chromatogram peak with a retention time of 69.18 min corresponding to the doublet CECdir-CECret.

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