Figure 5. Purification of doublet by RP-HPLC.
(a) RP-HPLC elution profile of the 80% ACN post Sep-Pak
C-18 with a gradient of 5-60% ACN.
(b) Protein profile resolved through
Tris-tricine / Urea (18%) gel electrophoresis Lane 1: Molecular Weight Standards
(Bio-Rad, CA, USA), Lane 2 eluate from chromatogram peak with a retention time
of 69.18 min corresponding to the doublet CECdir-CECret.