Electronic Journal of Biotechnology ISSN: 0717-3458
  © 2008 by Universidad Católica de Valparaíso -- Chile
Vol. 11 No. 2, Issue of April 15, 2008

Figure 2. Doublets design and sequence.
(a) Three-primer driven PCR reaction with hybrid primer (red-green) and flanking primers (red or green). DNA templates are in red and green boxes.
(b) CECdir-CECret (top panel) and CECret-CECdir (bottom panel) doublets construction design: using CECdir (in black) and CECret (in blue) as templates. Sequences of hybrid primers used for fusion are underlined in red. Initiation codon ATG is omitted from scheme.
(c) A scheme of the amino acid sequence of doublets of cecropin CECdir-CECret (top panel) and CECret-CECdir (bottom panel) from amino terminal to carboxi terminal orientation. Positively charged amino acids are marked by plus symbols (+). Initial methionine amino acid is omitted from scheme.

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