Electronic Journal of Biotechnology ISSN: 0717-3458
  © 2008 by Universidad Católica de Valparaíso -- Chile
Vol. 11 No. 2, Issue of April 15, 2008
 


Table 3. Immunological detection of CryIAb protein in soil samples.


Hybrid (event)

Date

CryIAb protein (pg / g soil)(*)

Pucará

(Control)

February 2002

29 ± 3

May 2002

30 ± 2

August 2002

12 ± 1

November 2002

16 ± 3

March 2003

10 ± 3

Pucará TD

(176)

February 2002

8 ± 3

May 2002

17 ± 3

August 2002

3 ± 2

November 2002

27 ± 4

March 2003

21 ± 2

30F82

(control)

February 2002

16 ± 2

May 2002

13 ± 3

August 2002

10 ± 1

November 2002

11 ± 3

March 2003

23 ± 2

32G63 MG

(MON810)

February 2002

16 ± 4

May 2002

9 ± 4

August 2002

9 ± 2

November 2002

4 ± 1

March 2003

37 ± 2

Three soil samples were obtained at invervals of three months. Total proteins were quantified by the Bradford method, and CryIAb protein was detected and quantified using a commercial ELISA kit (Envirologix), (*)mean ± SD.


  Supported by UNESCO / MIRCEN network