Electronic Journal of Biotechnology ISSN: 0717-3458
  © 2008 by Universidad Católica de Valparaíso -- Chile
Vol. 11 No. 2, Issue of April 15, 2008
 


Table 1. Total and CryIAb proteins rhizosecreted by transgenic maize events, and CryIAb protein content in root tissues after three weeks of hydroponic culture.


Hybrid
(Transgenic Event)

Total exudated
protein (ug/plant)(*)

CryIAb rhizosecreted
protein (pg/plant)

CryIAb protein in root
(ug/g fresh tissue)

 Chalten TD (176)

16.2 ± 1.31

ND

0.01 ± 0.001

 32G63MG (MON810)

23.7 ± 2.21

94 ± 5

1.56 ± 0.05

 Chalten TD max (BT11)

19.9 ± 2.03

19 ± 2

1.96 ± 0.04

 AX82 (Non- transgenic control)

21.19 ± 4.18

ND

NA

Three plantlets from each hybrid were sampled, and total proteins in the bag were quantified by the Bradford method. The CryIAb protein was quantified using a commercial ELISA kit (EnviroLogix) for Cry1A(b). Proper dilution of the exudated protein were prepared so that the CryIA protein fit into the calibration curve values (y = 3.2221x + 0.0015, R2 = 0.9998). CryIAb protein was also extracted and quantified from fresh root tissue. ND, not detectable; NA, not analyzed. (*) mean ± SD.


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