Electronic Journal of Biotechnology ISSN: 0717-3458
  © 2008 by Universidad Católica de Valparaíso -- Chile
Vol. 11 No. 1, Issue of January 15, 2008
 


Table 2. Number of T-DNA copies in 26 transgenic apple lines.


   Line   

 Detected T-DNA fragments 

Copy
 number 

 Loci 

 Presence of truncated T-DNA’s and 
transgene repeats

HindIII

XbaI

nptII

attE

nptII

attE

T311

1

1

2

3

3

1

truncated copies3,
transgene repeat

T315

8

2

8

6

8

8

truncated copies2, 3

T316

1

1

1

1

1

1

  

T320

2

1

2

2

2

2

  

T321

1

1

1

1

1

1

  

T322

3

1

3

3

3

3

  

T324

3

2

3

3

3

3

truncated copies2

T328

6

1

6

3

6

6

truncated copies3

T329

4

1

4

3

4

4

truncated copies3

T333

2

1

4

3

4

2

truncated copies3,
transgene repeats

T334

3

1

2

2

3

2

transgene repeat

T335

2

1

1

0

2

1

truncated copies3,
transgene repeat

T336

2

1

2

1

2

2

truncated copies3

T338

3

1

3

3

3

3

 

T340

2

1

2

1

2

2

truncated copies3

T344

1

1

n.a.

n.a.

11

11

 

T345

2

2

n.a.

n.a.

21

21

truncated copies2

T347

1

2

n.a.

n.a.

21

21

truncated copies2

T349

2

1

2

2

2

2

 

T353

7

3

5

5

7

5

truncated copies2,
transgene repeats

T355

2

2

2

2

2

2

truncated copies2

T357

1

1

n.a.

n.a.

11

11

 

T358

3

1

3

2

3

3

truncated copies3

T359

1

1

3

3

?

1

transgene repeat

T360

1

1

2

2

?

1

transgene repeat

T361

1

1

n.a.

n.a.

11

11

  

Pinova6

0

0

0

0

0

0

  

1Results are based on one Southern experiment - the number of copies is possibly higher.
2Truncated copies assumed after detection of multiple attE signals on HindIII digested DNA.
3Truncated copies assumed after detection of different numbers of nptII and attE signals on XbaI digested DNA.
6Non-transformed ‘Pinova’.
?: Results are ambiguous.
n.a.: not analysed.


Supported by UNESCO / MIRCEN network