Electronic Journal of Biotechnology ISSN: 0717-3458
  © 2008 by Universidad Católica de Valparaíso -- Chile
Vol. 11 No. 1, Issue of January 15, 2008
 


Table 1. Transgenic ‘Pinova’ apple lines: nptII and gusA activities in leaf tissue.


Genotype

nptII activity1

gusA activity4,5 of leaf number

First evaluation2

Second evaluation3

1

2

3

4

5

6

7

8

9

10

T311

47 ± 4.0

3 ± 0.2

1

3

2

3

2

1

1

2

2

4

T315

24 ± 0.9

17 ± 0.9

2

1

2

2

2

2

2

2

2

2

T316

29 ± 0.3

14 ± 0.3

2

2

1

1

1

1

1

1

1

1

T320

1 ± 0.0

7 ± 0.4

2

1

1

2

1

2

2

1

2

1

T321

7 ± 0.2

17 ± 0.5

5

5

5

5

5

5

5

5

5

5

T322

62 ± 5.0

120 ± 8.3

4

4

4

3

3

3

4

3

3

4

T324

31 ± 3.4

61 ± 13.6

3

3

3

4

3

3

4

5

3

3

T328

28 ± 1.1

10 ± 0.3

2

2

1

2

2

2

2

2

1

2

T329

30 ± 0.7

35 ± 2.0

3

5

2

5

5

5

4

5

4

4

T333

30 ± 1.1

92 ± 7.3

4

4

5

5

5

5

4

4

4

4

T334

49 ± 4.4

31 ± 2.2

4

4

4

4

4

4

4

4

4

4

T335

22 ± 1.7

21 ± 2.3

2

1

4

4

2

1

2

2

2

2

T336

18 ± 0.9

5 ± 0.2

4

4

4

4

4

4

2

2

4

5

T338

26 ± 1.1

16 ± 0.2

2

4

2

2

2

4

4

4

4

4

T340

27 ± 3.4

19 ± 1.7

4

4

5

4

4

4

4

4

4

4

T344

24 ± 4.4

11 ± 0.9

4

4

4

1

1

4

4

1

1

3

T345

13 ± 3.0

20 ± 2.2

1

1

2

2

3

1

2

3

3

2

T347

50 ± 1.5

37 ± 2.1

4

4

1

4

4

4

3

1

1

2

T349

36 ± 0.4

20 ± 1.1

1

1

1

1

2

1

4

1

1

1

T353

12 ± 0.4

46 ± 2.2

2

2

2

2

2

1

1

2

2

2

T355

2 ± 0.1

54 ± 0.9

1

1

1

1

1

1

1

1

1

1

T357

2 ± 0.0

1 ± 0.0

3

3

3

3

3

4

4

4

4

4

T358

2 ± 0.1

18 ± 0.6

2

1

2

1

2

2

1

1

2

1

T359

14 ± 2.8

54 ± 1.0

2

2

2

2

4

4

2

4

4

2

T360

9 ± 0.5

38 ± 2.1

2

1

1

1

2

2

1

2

1

2

T361

5 ± 0.1

1 ± 0.0

5

5

5

5

5

5

5

5

5

5

Pinova6

0.7 ± 0.0

0.6 ± 0.0

5

5

5

5

5

5

5

5

5

5

1NPTII protein in pg/mg fresh weight ± SD based on ELISA. The protein activity measurement was repeated three times for each protein sample.
2First evaluation was performed on transgenic lines after selection on medium containing selective antibiotics. 3Second evaluation was performed on transgenic lines after four years of subculture on medium without selective agents.
4Class of staining pattern for ten individual leaves in each transgenic line. Class 1: tissue completely dark blue or blue coloured; class 2: the major part (70-80%) of the tissue is blue, partially small white spots; class 3: tissue completely light blue coloured; class 4: the major part (70-80%) is white, partially small blue spots; class 5: tissue completely white.
5The GUS assay was performed on transgenic lines after four years of subculture on medium without selective agents.
6Non-transformed ‘Pinova’.


Supported by UNESCO / MIRCEN network