Electronic Journal of Biotechnology ISSN: 0717-3458
  © 2008 by Universidad Católica de Valparaíso -- Chile
Vol. 11 No. 1, Issue of January 15, 2008
 


Figure 8. Comparison of the real sequence of the apple cv. ‘Pinova’ and the sequence of the genomic T-DNA integration site in line T357. The real sequence was amplified using sequence-specific primers T357_F and T357R (bold letters) for genomic DNA of the non-transgenic genotype ‘Pinova’. The sequence of the integration site is based on information of the T-DNA flanking regions obtained after a Genome Walking procedure. Both sequences are nearly identical. Differences were found at the integration site (red coloured). A micro-homology was obtained between four bases of the genomic sequence and the first integrated bases of the T-DNA left border. Three bases (blue coloured) of the left border were truncated.


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