Electronic Journal of Biotechnology ISSN: 0717-3458
  © 2008 by Universidad Católica de Valparaíso -- Chile
Vol. 11 No. 1, Issue of January 15, 2008
 


Figure 7. PCR analysis of single shoots of the ‘Pinova’ transgenic apple line T357. Genomic DNA was extracted from leaf tissue of ten individual shoots:
(a) Using nptII specific primers;
(b) Using attE specific primers;
(c) Using the T-DNA specific primer pBI121_GW2 and the primer T357_F to amplify the right border adjacent region;
(d) Using the T-DNA specific primer pBI121_GW1 and the primer T357_R to amplify the left border adjacent region. MW 1 - 50 bp molecular weight marker, MW 2 - 100 bp molecular weight marker (both MBI Fermentas).


Supported by UNESCO / MIRCEN network