Electronic Journal of Biotechnology ISSN: 0717-3458
  © 2008 by Universidad Católica de Valparaíso -- Chile
Vol. 11 No. 1, Issue of January 15, 2008
 


Figure 2. Evaluation of attE gene transcription in transgenic apple lines by RT-PCR. The transcription of attE was evaluated directly after regeneration (a) and after four years of subculture on medium without antibiotics (b). The quality of the cDNA isolated from T-lines after four years of subculture was checked by RT-PCR using primers specific to Mal d3 (c). SM - molecular size marker 100 bp (MBI Fermentas); Plasm. - plasmid DNA.


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