Electronic Journal of Biotechnology ISSN: 0717-3458
  © 2007 by Universidad Católica de Valparaíso -- Chile
Vol. 10 No. 4, Issue of October 15, 2007
 


Table 2. ISSR primers used in the study and the number and the type of fragments they amplified.


Primer

Primer
sequence

Tm
(ºC)

bp

GC
(%)

Annealing
temp.
(ºC)

Single plant analysis

Bulk analysis

Fragment size
range (bp)

No. of
Scored
bands

No.of
polymorphic
bands

% of
polymorphic
bands

No.of
Scored
bands

No. of
polymorphic
bands

% of
polymorphic
bands

Single
plants

Bulk
plants

ISSR 1

(AGC)6-G

63.1

19

68.4

62

16

16

100

9

9

100

50-1600

50-1600

ISSR 2

(ACC)6-G

63.1

19

68.4

61

17

17

100

8

7

87.5

50-1000

50-800

ISSR 3

(AGC)6-C

63.1

19

68.4

62

10

7

70

9

6

66.66

50-1500

50-1500

ISSR 4

(CA)10-C

59.8

21

52.4

59

7

6

85.71

6

6

100

50-2300

50-1300

ISSR 5

(GA)9-C

56.7

19

52.6

56

11

11

100

8

8

100

50-1200

50-850

ISSR 6

GT-(CAC)7

67.8

23

65.2

66

12

12

100

8

7

87.5

50-930

50-930

ISSR 7

(AG)9-C

56.7

19

52.6

56

12

11

91.66

9

8

88.88

50-1000

50-1000

ISSR 8

(AC)9-G

56.7

19

52.6

56

12

12

100

6

6

100

50-2300

50-2300

ISSR 9

(AC)8-CG

56

18

55.6

55.5

11

11

100

14

14

100

50-1100

50-1250

ISSR 10

(AC)8-CC/T

54.8

18

52.8

55.5

19

19

100

15

13

86.66

50-1300

50-1000

ISSR 11

(CAC)5

53.3

15

66.7

52.5

-

-

-

2

0

0

-

300-750

ISSR 12

(GACAC)4

61.4

20

60

60.5

18

18

100

11

11

100

50-1300

50-1000

ISSR 13

(CA)6-A/GG

44.8

14

53.6

42.5

9

9

100

10

9

90

50-1200

50-1200

ISSR 14

(CA)6-RY

43.4

14

50

43

16

13

81.25

12

9

75

100-1100

100-2300

ISSR 15

(CA)8-AG

53.7

18

50

55.5

6

6

100

5

5

100

50-1250

50-950

ISSR 16

(CA)8-GC

56

18

55.6

55.5

18

16

88.88

11

9

81.81

50-1400

50-1000

ISSR 17

CAG-(CA)8

56.7

19

52.6

56

5

3

60

3

1

33.33

100-950

100-950

ISSR 18

CGT-(CA)8

56.7

19

52.6

56

6

5

83.33

7

5

71.42

100-780

50-1050