Electronic Journal of Biotechnology ISSN: 0717-3458
  © 2007 by Universidad Católica de Valparaíso -- Chile
Vol. 10 No. 3, Issue of August 15, 2007
 


Table 1. Optimised and experimental parameter values during batch bioreactor culture of Yarrowia lipolytica on stearin. Culture conditions: S0 = 20 mg/ml, pH = 6.0, incubation temperature T = 28ºC, fat/(NH4)2SO4 ratio 20 g/g, agitation rate 650 ± 20 rpm, aeration rate 0.3 v.v.m., oxygen saturation 60-70% (v/v) for all growth phases. Kinetics conducted in duplicate by using different inocula.


 

Verhlust

Blackman-Williams

Experimental
values

 μmax (h-1)

0.057

0.052

0.17

 YX/S(g/g)

1.38

1.25

1.56

 Xmax (mg/ml)

27.19

-

30.5

 a (IU/mg)

3.93 x 10-3

4.09 x 10-3

n.d.§

 KD [ml/(mg x h)]

3.93 x 10-4

3.63 x 10-4

n.d.§

 χ2

189

232

-

 χ2/D. F.

4.72

5.65

-

§: Non-determined.

 

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